Inhibition of Pseudomonas aeruginosa Biofilm Formation and Quorum Sensing System by Extracts of Prunus avium Stalk

Recently, misuse or overuse of antibiotics has led to the antibiotic resistance problem, a global healthcare problem. Most virulence factors and biofilm formation in Pseudomonas aeruginosa are controlled by quorum sensing (QS). The inhibition QS system inhibitor molecules been suggested as novel alternative antivirulence approach which no need kill bacteria. In present study, inhibitory potentials methanol acetone extracts Prunus avium stalk against P. were evaluated. tested at concentrations 240, 120, 60 μg/ml. lasB-gfp, rhlA-gfp, pqsA-gfp biosensor strains PAO1 used monitor inhibition, respectively. Fluorescence absorbance measurements performed on Cytation 3 multimode microplate reader. ratios for las, rhl, pqs systems recorded 70.43%, 47.25%, 76.31%, 47.76% (±6,60) 74.96%, 40.10%, 71.89%, 38.54% (±3,56) certain concentration 240 μg/ml, As result, anti-QS anti-biofilm properties better than that extracts. Further investigations needed discover compounds also their effects human cells then these may be new drug discoveries.